The comedian, aged 59, did not issue a full apology but acknowledged that he had shared unfounded rumors about the Princess before she disclosed her cancer diagnosis.
In a statement to his viewers on Monday night, Colbert stated that he does not intend to trivialize the Princess's situation.
He recognized the seriousness of a cancer diagnosis and extended well wishes to her for a swift recovery, acknowledging the emotional toll it can take on the patient and their loved ones.
Following the Princess's admission of cancer and undergoing preventive chemotherapy after abdominal surgery, speculation and conspiracy theories about her circulated online.
Colbert had previously commented on the issue, adding to the social media frenzy surrounding her whereabouts and condition.
Other celebrities like Kim Kardashian and Blake Lively also made light-hearted remarks about the Princess during this time but later expressed regret and apologized for their comments.
Actress Jamie Lee Curtis called for an end to the dissemination of conspiracy theories, emphasizing the importance of respecting the Princess's privacy and focusing on more significant societal issues.
Overall, the incident highlighted the need for sensitivity and caution when discussing personal matters, especially those related to health and well-being, regardless of one's public status or fame.